Immunohistochemical detection of progesterone receptors and the correlation with Ki-67 labeling indices in paraffin-embedded sections of meningiomas
International Journal of Neuroscience, cilt.112, sa.4, ss.463-470, 2002 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 112 Sayı: 4
- Basım Tarihi: 2002
- Doi Numarası: 10.1080/00207450290025581
- Dergi Adı: International Journal of Neuroscience
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.463-470
- Anahtar Kelimeler: immunohistochemistry, Ki-67, meningioma, progesterone receptor
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Female sex steroids may play a role in the proliferation of meningiomas, which usually have a high level of progesterone receptors (PgRs). We aimed to investigate the presence of PgRs and the Ki-67 labeling index (Ki-67 LI) in meningioma cases (N = 110) in relation to the severity of the disease. We studied PgR immunoreactivities and the Ki-67 LI in paraffin-embedded sections from meningiomas. Immunodetection of PgRs was conducted with peroxidase-antiperoxidase complex. Immunodetection of Ki-67 antigen was achieved by streptavidin-biotin-peroxidase complex. Of 110 meningiomas, in 57 (52%) the immunostaining for PgRs was moderately to strongly positive (Group 1), in 23 (20%) weakly positive (Group 2), and in 30 (28%) negative (Group 3). The positive immunostaining rate for the PgR in the benign meningiomas (76%) was significantly higher than that in nonbenign tumors. The positive immunostaining rate for the PgR was significantly higher in women (81%) than men (55%). The results suggested that progesterone may play a role in the growth of meningiomas. Our results confirmed that the immunodetection of the PgR and Ki-67 antigens on the paraffin sections of meningiomas provides a valuable tool for estimating the biological behavior of meningiomas.