Determination of atenolol in human urine by gas chromatography-mass spectrometry method
Journal of Chromatographic Science, cilt.49, sa.5, ss.365-369, 2011 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 49 Sayı: 5
- Basım Tarihi: 2011
- Doi Numarası: 10.1093/chromsci/49.5.365
- Dergi Adı: Journal of Chromatographic Science
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.365-369
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
A sensitive and efficient method was developed for the determination of atenolol in human urine by gas chromatography-mass spectrometry (GC-MS). Atenolol and metoprolol (internal standard, IS) were extracted from human urine with a mixture of chloroform and butanol at basic pH with liquid-liquid extraction. The extracts were derivatized with N-Methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) and analyzed by GC-MS using a capillary column. The standard curve was linear (r = 0.99) over the concentration range of 50-750 ng/mL. Intraand inter-day precision, expressed as the relative standard deviation were less than 5.0%, and accuracy (relative error) was beter than 7.0%. The analytical recovery of atenolol from human urine has averaged 91%. The limit of quantification was 50 ng/mL. Also, the method was successfully applied to a patient with hypertension who had been given an oral tablet of 50 mg atenolol.