Evaluation of air quality and microbial tests of the intravitreal injection room and the outpatient operating room İntravitreal enjeksiyon odası ile günübirlik ameliyathane odasının hava kalitesinin ve mikrobiyal yükünün değerlendirilmesi
Turk Hijyen ve Deneysel Biyoloji Dergisi, cilt.79, sa.1, ss.123-132, 2022 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 79 Sayı: 1
- Basım Tarihi: 2022
- Doi Numarası: 10.5505/turkhijyen.2022.65390
- Dergi Adı: Turk Hijyen ve Deneysel Biyoloji Dergisi
- Derginin Tarandığı İndeksler: Scopus, Academic Search Premier, CAB Abstracts, Veterinary Science Database, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.123-132
- Anahtar Kelimeler: air quality, günübirlik ameliyathene odası, hava kalitesi, Intravitreal injection room, microbial load, mikrobiyal yük, outpatient operating room, İntravitreal enjeksiyon odası
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objective: Intravitreal injections are one of the most commonly used treatment methods in ophthalmology, but they can cause serious infections that may result in vision loss. In our study, it was aimed to evaluate the particle, ventilation and microbial load of the intravitreal injection room (IVIR) and the outpatient operating room (OPR) where intravitreal injections were performed. Methods: Heating Ventilating and Air Conditioning (HVAC) performance test results for IVIR and OPR were examined. The air flow and flow rates of the filters were determined with a balometer device. For this measurement, hood suitable for filter sizes were used. The temperature and humidity of the environments were measured with a thermo-hygrometer device. Microbiological contamination was assessed by obtaining media cultures using the passive sample collection method. For this purpose, eosin methylene blue (EMB), blood agar and Sabouraud 2% dextrose agar medium petri dishes were left open for one hour on the table one meter above the ground and one meter away from the injection site. Colonies that reproduced after incubation were identified and similar colonies were counted. By using Omeliansky's formula to determine the microbial load per cubic meter of medium according to the number of colonies, the result was reported as colony forming units (cfu/m3). Results: The number of 0.5 pm particles per cubic meter was determined as 6,350,606 in the IVIR and 85,648 in the OPR. ACT and the “International Organization for Standardization” (ISO) class were found to be 2.0 and ISO 9 for IVIR, respectively, while 56.2 and ISO 7 for OPR. The bacterial density in the medium was found to be quite high as 484.86 cfu/m3 in IVIR, while it was found as 65.11 cfu/m3 in OPR. Staphylococcus epidermidis, Pantoea spp. and Enterobacter cloacae were grown in IVIR and OPR, and no fungal pathogens were detected. Conclusion: IVIR was found to be risky in terms of infection according to OPR. In intravitreal injections, it is very important to comply with the local antisepsis rules, keep the ultraviolet lamps on when the injection is not made, and have a high rate of air change per hour in the environments where the intraocular injection will be made. It will be much safer to make intravitreal injections in specially designed positive pressure rooms in order to prevent the development of infection.