Evaluation of Bioremediation and Genoprotective Properties of Pseudomonas sp. SS5 Isolated from the Terrestrial Isopods (Isopoda: Oniscidea)
Soil and Sediment Contamination, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Basım Tarihi: 2026
- Doi Numarası: 10.1080/15320383.2026.2673897
- Dergi Adı: Soil and Sediment Contamination
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, Compendex, Environment Index, Academic Search Ultimate (EBSCO), Natural Science Collection (ProQuest), Biomedical Reference Collection: Corporate Edition (EBSCO), Earth, Atmospheric, & Aquatic Science Collection (ProQuest), Engineering Source (EBSCO), Materials Science & Engineering Collection (ProQuest), Pharma Collection (ProQuest), Technology Collection (ProQuest)
- Anahtar Kelimeler: Bacteria, bioremediation, isolation, pill bug, Pseudomonas
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
This study aims to identify a bacterium for heavy metal bioremediation. Pill bugs (Crustacea; Isopoda; Oniscidea), known for their ability to accumulate and detoxify heavy metals in humid environments, were chosen as the bacterial source. A bacterium was isolated from pill bugs and identified by 16S rDNA sequencing. Its heavy metal removal capacity from liquid culture medium was then evaluated using ICP-MS. Lastly, the therapeutic and protective effect of the isolate against heavy metal-induced genotoxicity was evaluated at the chromosomal level in the model organism Allium cepa, focusing on the heavy metal most effectively removed by the bacterium. As a result of the study, a bacterium identified as Pseudomonas sp. SS5. The bacterium’s bioremediation capacity was assessed over 24h and 48h, with the following removal percentages observed: iron (6.07% and 24.38%), copper (17.82% and 63.29%), cadmium (0.41% and 4.11%), aluminum (12.29% and 30.73%), nickel (0.76% and 0.58%), and manganese (0% at 24h but 41.05% at 48h). The bacterium exhibited the highest removal efficiency for copper. In pre-, co-, and post-treatment groups of A. cepa, the bacterium showed protective effects against copper-induced mutagenicity at all tested concentrations. The SS5 strain significantly reduced copper-induced chromosomal aberrations and DNA damage compared to the control.