Is montelukast effective in regression of endometrial implants in an experimentally induced endometriosis model in rats?

Kiykac Altinbas S., Tapisiz O. L., Cavkaytar S., ŞİMŞEK G., Oguztuzun S., Goktolga U.

European Journal of Obstetrics and Gynecology and Reproductive Biology, cilt.184, ss.7-12, 2015 (SCI-Expanded, Scopus)

Özet

Objective Montelukast, a selective antagonist of Type 1 cysteinyl leukotriene receptors (CysLT 1 Rs), antagonizes the proinflammatory and proasthmatic activities of CysLT 1 Rs. We investigated the effect of montelukast on a surgically induced endometriosis rat model. Study design Thirty-two sexually mature, cycling, female Wistar-Albino rats, in which endometriotic implants were surgically induced, were randomly divided into three groups. Group I [Montelukast (M), 10 rats)] was given 1.6 mg/kg/day of oral montelukast sodium. Group II [Leuprolide acetate (L), 11 rats] was given 1 mg/kg single dose of s.c.leuprolide acetate. Group III [Control (C), 11 rats] received saline solution through an orogastric tube and served as controls. After a 3-weeks medication, the rats were sacrificed to investigate the endometriotic implants for size and morphological and histological characteristics, including immunoreactivity of MMP-2 and VEGF. Results The mean area of implants decreased from 48.2 ± 24.7 to 29.3 ± 15.8 mm 2 in Group I (M) (P = 0.008) and from 62 ± 32.1 to 39.9 ± 18.1 mm 2 in Group II (L) (P = 0.003). In Group III (C), the mean area increased from 41.1 ± 31.1 to 60.4 ± 37.1 mm 2 (P = 0.025). Histopathological analysis showed statistically significant lower scores in rats treated with montelukast compared to leuprolide and controls. MMP H scores were not different between the groups in both epithelial and stromal MMP-2 immunostaining. VEGF H scores were statistically lower in Group 1 (M) in epithelial VEGF immunostaining when compared to Group II (L) and Group III (C) (P = 0.006). Conclusion(s) Montelukast may effectively cause a significant decrease in the area of endometriotic implants.