Genotypic characteristics of neisseria meningitidis serogroup w-135 strains isolated as the agent of fatal meningitis in a military hospital Bir askeri hastanede fatal menenjit etkeni olarak izole edilen neisseria meningitidis serogrup w-135 suşlarinin genotipik özellkleri
Mikrobiyoloji Bulteni, cilt.43, sa.3, ss.515-517, 2009 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Derleme
- Cilt numarası: 43 Sayı: 3
- Basım Tarihi: 2009
- Dergi Adı: Mikrobiyoloji Bulteni
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.515-517
- Anahtar Kelimeler: Fatal meningitis, Military recruits, Neisseria meningitidis, Serogroup w- 7 35, Turkey
- Sağlık Bilimleri Üniversitesi Adresli: Hayır
Özet
The aim of this study was to describe the genetic characterization of a total of 6 Neisseria meningitidis serogroup W-135 strains isolated from patients with meningitis and carriers in a military hospital in 2007-2008. Suspected colonies on modified Thayer-Martin medium plates were screened for oxidase reactivity and Gram stain. If gram-negative diplococci were present, a biochemical profile by the API NH system was used for species confirmation. Pulse field gel electrophoresis typing of Nhei-digested DNA was performed by a previously described method. Multi-locus sequence typing (MLST) was performed using the standard primers as listed on the Neisseria MLST website. Three distinct sequence types (STs) were identified: ST-11, ST-2754, ST-3751. One of the clinical isolates was identified as the same sequence type with Hajj isolate (ST-11) and the isolate with ST-2754 was the same as the first Turkish clinical strain isolated in 2003. These data demonstrated that along with ST-11 which is a known Hajj isolate, the ST-2754 strain causing meningococcal disease in Turkey beginning from the year 2003, should be carefully monitored.