The effect of ozone treatment on experimental colon anastomosis in rats
European Surgery - Acta Chirurgica Austriaca, cilt.48, sa.2, ss.122-128, 2016 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 48 Sayı: 2
- Basım Tarihi: 2016
- Doi Numarası: 10.1007/s10353-015-0372-2
- Dergi Adı: European Surgery - Acta Chirurgica Austriaca
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.122-128
- Anahtar Kelimeler: Colon anastomosis, Ozone therapy, Oxidative stress, Preconditioning
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Background: Colonic anastomosis is a common surgical procedure. Complications after such surgery are still a major problem despite advances in techniques. We aimed to evaluate the effects of pre-operative and postoperative ozone administration on the recovery of colonic anastomoses in rats in this study. Methods: An end-to-end colon anastomosis was created in the left colon under anesthesia in all experiment group animals. The control group was left alone for 4 days. The other two groups were administered intraperitoneal ozone at a dose of 1 mg/kg once a day. Ozone therapy was started 24 h after surgery to one group (ozone) and 1 h before surgery to the other group (pre-ozone). Samples for biochemical measurements were obtained from the operation site after adhesion scoring was performed and the burst pressure measured. Results: The burst pressure and OH-proline measurements in the ozone group were found to be significantly higher than in the other groups (p ˂ 0.05). These values were not different between the pre-ozone and control groups (p ˃ 0.05). The MDA values of the ozone group were significantly lower but the antioxidant enzyme levels (except superoxide dismutase) were significantly higher than the other two groups. The TNF-α and IL-6 levels of the ozone group were significantly lower while the IL-10 levels were significantly higher compared with the other two groups. Conclusion: We therefore feel that ozone treatment after surgery could be important in terms of accelerating colon anastomosis recovery. Ozone administration supports recovery by reducing oxidative stress and proinflammatory cytokines.