Assessment of Anticancer Properties of Synthesized Pyrazole-Acridine Derivative on SH-SY5Y Human Neuroblastoma Cells Sentezlenen Pirazol-Akridin Türevinin SH-SY5Y İnsan Nöroblastoma Hücrelerinde Antikanser Özelliklerinin Değerlendirilmesi
Duzce Medical Journal, cilt.27, sa.1, ss.1-10, 2025 (ESCI, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 27 Sayı: 1
- Basım Tarihi: 2025
- Doi Numarası: 10.18678/dtfd.1520993
- Dergi Adı: Duzce Medical Journal
- Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, CINAHL, EMBASE, Directory of Open Access Journals, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.1-10
- Anahtar Kelimeler: Chemical synthesis, antineoplastic agents, neuroblastoma, pyrazoles, acridines, cytotoxicity, apoptosis
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Aim: This study aimed to synthesize a novel pyrazole acridine derivative (3-ACH) and evaluate its anticancer properties on SH-SY5Y human neuroblastoma cells. Material and Methods: The pyrazole-4-carbaldehyde derivative was cyclized with dimedone and p-nitroaniline to synthesize the 3-ACH. Characterization of the compound was performed using FT-IR, NMR, HPLC-Q-TOF/MS, and elemental analysis. The cytotoxic impact of the 3-ACH compound on SH-SY5Y human neuroblastoma cells was evaluated using the WST-1 assay in a dose-(50, 100, and 150 μg/mL) and time-dependent (12, 24, and 48 hours) manner. The impact of 3-ACH on apoptosis was investigated through immunostaining for Caspase-3, -8, and -9, and BAX proteins. Results: 3-ACH reduced SH-SY5Y cell viability with the rate of 89.89±7.63% (p=0.002) at 100 μg/mL concentration after 24 hours of treatment. While a higher (150 μg/mL) concentration showed a similar reduction (90.53±2.88%, p=0.004), the lower (50 μg/mL) concentration maintained high cell viability (98.37±1.67%, p=0.903) at 24 hours. All doses of 50 μg/mL (94.55±0.65%), 100 μg/mL (95.18±1.41%), and 150 μg/mL (95.28±2.57%) significantly reduced cell viability rates, at 48 hours (all p< <0.001). Immunostaining revealed a significant upregulation in the synthesis of BAX, Caspase-3, -8, and -9 proteins in cells treated with 3-ACH (100 μg/mL) for 24 hours compared to the control group. Conclusion: These results indicate that 3-ACH has the potential to induce apoptosis in SH SY5Y cells by activating both the intrinsic and extrinsic pathways, which may contribute to its cytotoxic effects. This study provides promising evidence supporting the potential of the 3 ACH compound as an anticancer therapeutic agent.