Protective effects of oleuropein isolated from Olea europaea L. on renal ischemia/reperfusion injury in rats: Insights into antioxidant, and anti-apoptotic pathways
Phytomedicine, cilt.150, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 150
- Basım Tarihi: 2026
- Doi Numarası: 10.1016/j.phymed.2025.157691
- Dergi Adı: Phytomedicine
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CINAHL, EMBASE, MEDLINE, Natural Science Collection (ProQuest), Health Research Premium Collection (ProQuest)
- Anahtar Kelimeler: Oleuropein, Ischemia/reperfusion injury, Kidney, Oxidative stress, Apoptosis, Rat model
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Aim This study aimed to evaluate the protective effects of oleuropein, a compound recognised for its antioxidant and anti-apoptotic properties, against renal ischemia/reperfusion injury (IRI) in rats. Materials and Methods Oleuropein, purified from Olea europaea L. (olive tree) by chromatographic methods, was injected intraperitoneally 48 h, 24 h, and 30 min prior to ischemia. Forty male Sprague-Dawley rats were randomly divided into five groups: Control, IRI, and three treatment groups subjected to IRI and treated with oleuropein at 10, 50, and 100 mg/kg, respectively. In all IRI groups, 45 min of bilateral renal ischemia was followed by 24 h of reperfusion. Biochemical analyses included total antioxidant status (TAS), total oxidant status (TOS), superoxide dismutase (SOD), and paraoxonase-1 (PON-1) levels in serum and kidney tissues. Renal function was assessed by serum creatinine, urea, and uric acid levels. Apoptotic markers, including cytochrome c and caspase-3, were evaluated by Western blotting and immunofluorescence. Results In the IRI group, TAS and SOD levels significantly decreased, whereas TOS and PON-1 increased (p<0.05). Oleuropein administration improved these parameters in a dose-dependent manner. At 50 mg/kg, oxidative stress markers and renal function tests approached control values (p<0.001). Apoptotic markers were elevated in the IRI group but markedly reduced after oleuropein treatment. Conclusion Oleuropein protected kidney tissue against IRI by reducing oxidative stress and apoptosis. These results suggest that oleuropein may serve as a potential therapeutic agent against renal IRI.