Biochemical analysis of microbiotas obtained from healthy, prediabetic, type 2 diabetes, and obese individuals
Turkish Journal of Biochemistry, cilt.48, sa.1, ss.58-65, 2023 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 48 Sayı: 1
- Basım Tarihi: 2023
- Doi Numarası: 10.1515/tjb-2022-0110
- Dergi Adı: Turkish Journal of Biochemistry
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Food Science & Technology Abstracts, Directory of Open Access Journals, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.58-65
- Anahtar Kelimeler: microbiota, obesity, prediabetes, real-time qPCR, type 2 diabetes
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objectives: In this study, we aimed to evaluate the intestinal and urinary microbiota diversity of obese, pre-diabetic, diabetic, and healthy subjects together with their food consumption frequency and investigate the effect on glucose metabolism. Methods: DNA was isolated from stool and urinary samples of fifteen obese, fifteen prediabetics, fifteen type 2 diabetic, and fifteen lean participants by using the quantitative real-time polymerase chain reaction (qPCR) method. The amounts of Bifidobacterium, Bacteroides, and Firmicutes were measured and food consumption frequency was answered by all participants through a questionnaire. Results: The levels of Bifidobacterium in fecal microbiota were significantly higher in type 2 diabetic patients compared with lean (p=0.034), prediabetic (p=0.009), and obese participants (p=0.012). However, the levels of Bifidobacterium in urinary microbiota were decreased in obese, prediabetic, and type 2 diabetic subjects as controls (p=0.048; p=0.038; p=0.015 respectively). Additionally, Bacteroides/Firmicutes ratio decreased in type two diabetic patients compared with lean subjects and had a negative correlation with BMI in prediabetic subjects. Food consumption frequency illustrates that lean subjects have unhealthy eating habits. Conclusions: Urinary microbiota could be considered in the future context of a potential biomarker in the progress of type 2 diabetes and obesity.