The inhibitory role of melatonin on isolated guinea-pig urinary bladder: An endogenous hormone effect
BJU International, cilt.94, sa.9, ss.1373-1376, 2004 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 94 Sayı: 9
- Basım Tarihi: 2004
- Doi Numarası: 10.1111/j.1464-410x.2004.05175.x
- Dergi Adı: BJU International
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1373-1376
- Anahtar Kelimeler: bladder, detrusor, contraction, melatonin, inhibition
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
OBJECTIVE: To investigate the effects of melatonin, an endogenous hormone, on acetylcholine and KCl-induced contractions of isolated guinea-pig detrusor muscle. MATERIALS AND METHODS: Detrusor smooth muscle strips isolated from guinea-pig bladders were placed in an organ bath containing physiological saline at 37°C and pH 7.4, constantly bubbled with 95% oxygen and 5% CO 2. The effects of cumulatively applied melatonin on the acetylcholine- and KCl-induced contractions of isolated bladder strips were examined using isometric contraction measurements. RESULTS: Melatonin (100 and 300 μmol/L) significantly inhibited the peak amplitude of both acetylcholine (10 μmol/L) and KCl (30 mmol/L)-induced contraction of the isolated bladder strips (P < 0.05). Similarly, melatonin caused a significant reduction in the contractile frequency induced by KCl (eight strips) in a concentration- dependent manner, while having no significant effect on the frequency of contractile response to acetylcholine, even at the highest concentration (300 μmol/L used (P = 0.58, 14 strips). CONCLUSIONS: These results suggest that melatonin inhibits acetylcholine- and KCl-induced contractions in isolated bladder strips from guinea pigs. The endogenous nature of melatonin, with its low side-effect profile, makes it a potentially useful agent to be considered in the medical management of the overactive bladder.