The antioxidant and antigenotoxic properties of citrus phenolics limonene and naringin

GÜDÜL BACANLI M., BAŞARAN A. A., Başaran N.

Food and Chemical Toxicology, cilt.81, ss.160-170, 2015 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 81
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1016/j.fct.2015.04.015
  • Dergi Adı: Food and Chemical Toxicology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.160-170
  • Anahtar Kelimeler: Limonene, Naringin, Trolox equivalent antioxidant capacity, MTT, COMET, Micronucleus
  • Sağlık Bilimleri Üniversitesi Adresli: Hayır

Özet

Phenolic compounds not only contribute to the sensory qualities of fruits and vegetables but also exhibit several health protective properties. Limonene and naringin are the most popular phenolics found in Citrus plants. In this study, we investigated the antioxidant capacities of limonene and naringin by the trolox equivalent antioxidant capacity (TEAC) assay and the cytotoxic effects by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in Chinese hamster fibroblast (V79) cells. The genotoxic potentials of limonene and naringin were evaluated by micronucleus (MN) and alkaline COMET assays in human lymphocytes and V79 cells. Limonene and naringin, were found to have antioxidant activities at concentrations of 2-2000 μM and 5-2000 μM respectively. IC50 values of limonene and naringin were found to be 1265 μM and 9026 μM, respectively. Limonene at the concentrations below 10,000 μM and naringin at the all concentrations studied, have not exerted genotoxic effects in lymphocytes and in V79 cells. Limonene and naringin at all concentrations revealed a reduction in the frequency of MN and DNA damage induced by H2O2.