Diagnostic accuracy and post-reperfusion kinetics of serum miRNA-125b in hyperacute ischemic stroke: a prospective emergency department case–control study
Irish Journal of Medical Science, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s11845-026-04536-3
- Dergi Adı: Irish Journal of Medical Science
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, MEDLINE, Biomedical Reference Collection: Corporate Edition (EBSCO), Health Research Premium Collection (ProQuest)
- Anahtar Kelimeler: Acute ischemic stroke, MiRNA-125b, MiRNA-124, Reperfusion therapy, Diagnostic accuracy
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Background: The utility of circulating microRNAs as early diagnostic biomarkers in the hyperacute phase of acute ischemic stroke (AIS) remains under investigation. Aims: To evaluate the diagnostic accuracy of serum miRNA-124 and miRNA-125b at emergency department (ED) admission and to characterize their dynamic changes following acute reperfusion therapy. Methods: This prospective, single-center, case-control study included adult patients with AIS in the hyperacute phase, defined as presentation within 6 h of symptom onset, who were treated with reperfusion therapy (intravenous thrombolysis and mechanical thrombectomy), along with age- and sex-matched healthy controls. Serum samples were collected at baseline (pretreatment) and 24 h post-reperfusion, and miRNA levels were quantified using quantitative real-time polymerase chain reaction. Diagnostic performance was evaluated using receiver operating characteristic curve analysis. Results: In total, 20 patients and 10 controls were included. At baseline, serum miRNA-125b levels were significantly elevated in AIS patients compared to controls (p < 0.001), demonstrating high diagnostic accuracy with an area under the curve of 0.910, 95.0% sensitivity, and 90.0% specificity. In contrast, miRNA-124 exhibited no significant difference (p = 0.266) and lacked discriminative value. At 24 h post-reperfusion, miRNA-125b levels exhibited a nonsignificant downward trend (p = 0.108). Neither miRNA correlated with baseline stroke severity (NIHSS) or early neurological status (mRS). Conclusions: Serum miRNA-125b showed promising preliminary diagnostic performance for AIS at ED admission. A single 24 h post-reperfusion measurement exhibited a nonsignificant decline in miRNA-125b and no association of miRNA with early stroke severity or functional outcome.