The effect of post-contrast washıng on post-endoscopıc retrograde cholangıopancreatography pancreatıtıs
International Journal of Clinical and Experimental Medicine, cilt.9, sa.8, ss.15868-15875, 2016 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 9 Sayı: 8
- Basım Tarihi: 2016
- Dergi Adı: International Journal of Clinical and Experimental Medicine
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.15868-15875
- Anahtar Kelimeler: Post ERCP pancreatitis, experimental pancreatitis, contrast induced pancreatitis
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objective: We aimed to evaluate the efficacy of dilutional washing with saline solution following the administration of contrast agent for ERCP in decreasing the rate of pancreatitis, in our experimental rat model. Methods: Fourty Wistar-Albino® male rats of 250-300 g were divided into 4 equal groups. Group 1: Cannulation, Group 2: Cannulation+saline, Group 3: Cannulation+contrast agent, Group 4: Cannulation+contrast agent+saline. At the 24th hour following the procedures, the rats were sacrified and pancreatic tissues were examined histopathologi-cally, with the evaluation of blood levels of leukocyte, glucose, (SGOT), (LDH), amylase, C-reactive protein (CRP) and acid-base status. Histopathological grading of acute pancreatitis was performed using haematoxylin and eosin staining. Results: Mean levels of amylase, leukocyte, AST and LDH were found to be significantly higher in groups 2, 3, 4 when compared to group 1 (P<0.05). CRP level was found to be highest in group 3 (P>0.05). Histopathological grade of pancreatitis was found to be significantly higher in groups 2, 3, 4 when compared to group 1 (P<0.05). Scores of edema, acinar necrosis and amylase level were found to be higher in group 3 than group 4. Scores were similar in groups 2 and 4. Conclusion: We found that dilution with saline solution during ERCP procedure may be beneficial in decreasing the rate of post ERCP pancreatitis, which is shown with histopathological and laboratory findings. We suggest dilutional washing out of the contrast agent at low pressures following the ERCP procedure.