The antioxidant capacity and in-vitro anticancer effects of Artocarpus lakoocha Roxb. against laryngeal cancer cells
Scientific Reports, cilt.16, sa.1, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 16 Sayı: 1
- Basım Tarihi: 2026
- Doi Numarası: 10.1038/s41598-026-38501-y
- Dergi Adı: Scientific Reports
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, EMBASE, MEDLINE, Directory of Open Access Journals, Zoological Record, Academic Search Ultimate (EBSCO), Natural Science Collection (ProQuest), Biological Science Database (ProQuest), Biomedical Reference Collection: Corporate Edition (EBSCO), Health Research Premium Collection (ProQuest)
- Anahtar Kelimeler: Laryngeal cancer, <italic>Artocarpus lakoocha</italic> Roxb., Antioxidant, Cytotoxicity, Proapoptotic, Antimigrative
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Laryngeal cancer has a poor prognosis and invasive character. Despite recent technological advances, laryngeal cancer remains challenging with a low survival rate. Artocarpus lakoocha Roxb. (AL) is rich in oxyresveratrol with antioxidant, cytotoxic, and anti-inflammatory properties This study aimed to determine the total phenolic content, antioxidant capacity and in-vitro anticancer effects of AL on laryngeal cancer cell lines. Total phenolic content and antioxidant activity of AL was evaluated using Folin-Ciocalte and DPPH tests. Cytotoxic and antiproliferative effects were measured with MTT and live-dead assays, while CASP-3, BAX, and BCL-2 expressions were analyzed via qRT-PCR and western blotting. Cell migration inhibition was determined by scratch-wound healing assay. The total phenolic content of AL was calculated as 541.3 ± 14.1 mg gallic acid equivalent (GAE)/g. The IC50 value for the scavenging effect of AL against DPPH radical was 98.03 ± 0.5780 µg/mL. AL demonstrated dose- and time-dependent in-vitro cytotoxic effects on Hep-2 and SCC-90 cells and exhibited higher selectivity toward cancer cells than Detroit-551 fibroblasts. Microscopic imaging confirmed that AL caused a dose-dependent decrease in cell viability and a dose-dependent cancer cell death. AL also inhibited migration of cancer cell lines and modulated apoptosis related genes and proteins by upregulating CASP-3 and BAX while downregulating BCL-2. Overall, the high phenolic content, antioxidant capacity and observed in-vitro anticancer effects of AL support its consideration as a biologically active natural compound for further investigation in laryngeal cancer.