Refined cuff technique minimizes surgical complexity in rat lung transplantation and improves outcomes
Scientific Reports, cilt.15, sa.1, 2025 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 15 Sayı: 1
- Basım Tarihi: 2025
- Doi Numarası: 10.1038/s41598-025-19711-2
- Dergi Adı: Scientific Reports
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, MEDLINE, Directory of Open Access Journals
- Anahtar Kelimeler: Lung transplantation, Cuff technique, Model modification, Rat, Anastomosis
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Orthotopic lung transplantation in rats serves as a pivotal platform for exploring long-term allograft outcomes, yet technical complexity has limited its widespread application. Here, we present a refined surgical model that combines structured intraoperative modifications with tailored mechanical ventilation strategies to ensure procedural consistency and biologic fidelity. Key interventions—such as a custom leak-proof endotracheal tube, a temporally controlled reperfusion maneuver, and an optimized anastomotic sequence—collectively addressed critical challenges including airway obstruction, graft impaired perfusion, and venous fragility. The protocol demonstrated a high procedural success rate and reproducible long-term survival, with histopathological analysis confirming progressive chronic remodeling and diminished acute rejection. Importantly, the technique demonstrated features that facilitate rapid acquisition, supported by a short learning curve, and was implemented using widely accessible clinical-grade materials. Together, these attributes establish a robust and scalable platform for investigating chronic lung allograft dysfunction and other long-term immunologic phenomena.