Evaluation of Serum Interleukin-33 and Gene Polymorphisms in Patients with Bronchial Asthma
Gulhane Medical Journal, cilt.63, sa.2, ss.104-109, 2021 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 63 Sayı: 2
- Basım Tarihi: 2021
- Doi Numarası: 10.4274/gulhane.galenos.2020.1438
- Dergi Adı: Gulhane Medical Journal
- Derginin Tarandığı İndeksler: Scopus, Academic Search Premier, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.104-109
- Anahtar Kelimeler: Asthma, cytokine, IL-33
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Aims: Asthma is a chronic inflammatory disease of the airways. The aim of the study was to investigate the association between serum interleukin (IL)-33 level and IL-33 gene rs1342326, rs1929992 and rs3939286 polymorphisms in patients with asthma. Methods: Ninety patients aged 18 years and older, who were diagnosed with asthma, and 90 healthy control subjects were included in the study. Serum IL-33 levels were measured by enzyme-linked immunosorbent assay; and IL-33 gene polymorphisms were studied by realtime polymerase chain reaction. Results: IL-33 level was higher in patients with asthma (4.16±0.88 pg/mL) compared to the control subjects (1.28±0.37 pg/mL) (p<0.001). The frequencies of AA, CC and AC alleles for the rs1342326 polymorphism were 52 (57.8%), 5 (5.6%) and 33 (36.7%) in the patient group, and 53 (58.9%), 7 (7.8%) and 30 (33.3%) in the control group, respectively. The frequencies of CC, TT and CT alleles for the rs1929992 polymorphism were 17 (18.9%), 31 (34.4%), and 42 (46.7%) in the patient group and 13 (14.4%), 41 (45.6%), and 36 (40%) in the control group, respectively. The frequencies of CC, TT and CT alleles for the rs3939286 polymorphism were 46 (51.1%), 7 (7.8%), and 37 (41.1%) in the patient group and 44 (48.9%), 9 (10%), and 37 (41.1%) in the control group, respectively. There was no statistically significant difference between the groups in terms of IL-33 gene polymorphisms (rs1342326, rs1929992 and rs3939286) (p=0.784, p=0.304, p=0.863, respectively). Conclusions: The current study showed increased serum IL-33 levels in patients with asthma compared to healthy controls but gene polymorphism studies did not show any significant difference.