The comparative results of three different real-time PCR assays: MY09/11 primers failed to detect multiple infections Üç farkli real-time PCR testinin karşilaştirmali sonuçlari: MY09/11 primerleri çoklu enfeksiyonlari kaçiriyor
Gulhane Medical Journal, cilt.56, sa.2, ss.65-70, 2014 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 56 Sayı: 2
- Basım Tarihi: 2014
- Doi Numarası: 10.5455/gulhane.41476
- Dergi Adı: Gulhane Medical Journal
- Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.65-70
- Anahtar Kelimeler: HPV, Multiple infection, MY09/11, Real-time PCR
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
HPV-DNA testing is widely used worldwide today and it has become an important part of cervical cancer screening programs. The aim of this study is re-evaluating the effectiveness of MY09/11 consensus primer system, which is one of the most widely used HPVDNA tests, by using a different approach. In this study, MY09/11 consensus PCR and two different TaqMan-based type-specific PCR assays were used for investigation of the presence of 17 different HPV types in cervical smear samples. Of the 470 samples, 33.2% (156/470) were HPV-DNA positive by at least one of the three methods and remains were negative by all methods. A total of 220 different HPV isolates were identified in 149 samples by the type specific PCR, while the nine samples were positive by consensus PCR only. A single HPV type was detected in 64.4% (96/149) of the genotyped samples, and multiple HPV types were detected in the remaining 35.6% (53/149). MY09/11 PCR failed to detect 21.2% (33/156) of all HPV-DNA positive samples and the rates of false negative results were 18.75% (18/96) and 28.3% (15/53) in single and multiple infections, respectively. We conclude that highly sensitive type-specific PCR tests may be a useful tool for screening of cervical cancer. © Gülhane Askeri Tip Akademisi 2014.