Impact of calcium propionate on cellular behavior in A549 and DMS114 lung cancer cell lines
Turkish Journal of Biochemistry, cilt.49, sa.5, ss.666-673, 2024 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 49 Sayı: 5
- Basım Tarihi: 2024
- Doi Numarası: 10.1515/tjb-2024-0054
- Dergi Adı: Turkish Journal of Biochemistry
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.666-673
- Anahtar Kelimeler: calcium propionate, cancer, food additive, cell viability, lung cancer
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objectives: To investigate the cellular and apoptotic effects of food additive calcium propionate by in vitro methods. Methods: Cell viability by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, intracellular Glutathione (GSH) by luminometric method, mitochondrial membrane potential (MMP) by fluorometry, apoptosis by dual Acridine Orange/Ethidium Bromide (AO/EB) fluorescent staining were studied in lung cells. Results: It has been observed that calcium propionate reduced GSH and MMP levels significantly while increased cytotoxicity, apoptosis, and reactive oxygen species (ROS) levels in both A549 and DMS114 cancer cells in a dose-dependent manner. Conclusions: Cytotoxic effects of calcium propionate were more pronounced in cancer cells compared to healthy cells, suggesting its potential suitability as a chemo-preventive agent.