Effect of octenidine dihydrochloride on viability of protoscoleces in hepatic and pulmonary hydatid diseases
Journal of the National Medical Association, cilt.99, sa.6, ss.674-677, 2007 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 99 Sayı: 6
- Basım Tarihi: 2007
- Dergi Adı: Journal of the National Medical Association
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.674-677
- Anahtar Kelimeler: dihydrochloride, liver, lung
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Background: Use of effective scolicidal agents during puncture, aspiration or injection of a scolicidal agent and reaspiration (PAIR) and surgery for hydatid cysts are essential to reduce the recurrence rate. In this in vitro study, we tried to determine the scolicidal property of a new agent, octenidine dihydrochloride, and of various agents in different concentrations and exposure times. Material and Methods: Echinococcus granulosus protoscoleces were obtained from six patients with liver (n=3) and lung (n=3) hydatid cysts. Various concentrations of octenidine dihydrochloride (0.1%, 0.01% and 0.001% diluted form), povidone iodine (10%, 1% and 0.1% diluted) and 20% saline were used in this study. Viability of protoscoleces was determined with dye-uptake (0.1% eosin) and flame cell activity. Results: Octenidine dihydrochloride 0.1% had strong scolicidal effect in 15 min and octenidine dihydrochloride 0.01% in 30 min. Sixty percent of protoscoleces lost viability at 5 min with octenidine dihydrochloride 0.1%. Viability ratio decreased to 20% at 10 min, and all of them died at 15 min. Povidone iodine 10% and 1% had strong scolicidal effects after 15- and 30 min of exposure, respectively. Saline 20% killed all the protoscoleces in 30-min exposure. Conclusion: Because of the rapid and strong scolocidal effectiveness of octenidine dihydrochloride on protoscoleces, it may be used as a scolocidal agent during both perioperative and in the PAIR method.