Toluidine blue staining in the diagnosis of endometrial pathologies: A preliminary study before chromohysteroscopy
European Journal of Obstetrics and Gynecology and Reproductive Biology, cilt.160, sa.2, ss.191-195, 2012 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 160 Sayı: 2
- Basım Tarihi: 2012
- Doi Numarası: 10.1016/j.ejogrb.2011.10.013
- Dergi Adı: European Journal of Obstetrics and Gynecology and Reproductive Biology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.191-195
- Anahtar Kelimeler: Toluidine blue dye, Endometrium staining, Diagnosis of endometrial cancer, Chromohysteroscopy, Frozen section procedure
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objective: To assess the efficacy of toluidine blue (TBlue) staining in the detection of endometrial premalignant or malignant lesions in uterine specimens obtained from hysterectomies. We hoped that the results of this preliminary study would help us in our future studies which may be on chromohysteroscopy. Study design: We developed an endometrium staining technique in which TBlue solution was used as a vital dye. The study enrolled 50 patients. Of these, 20 patients known to have a premalign or malign lesion of the uterus constituted the study group and 30 patients were recruited as the control group who were operated for leiomyoma of the uterus. Immediately after hysterectomy, all specimens were dyed with TBlue solution and a frozen section procedure was performed on the positively stained areas. The pre- and post-operative histopathological diagnoses of the participants were compared and the reliability of the new method was calculated. Results: The sensitivity of TBlue staining in the determination of endometrial pathologies was 100% (95%, CI 0.83-1) with a specificity of 90% (95%, CI 0.74-0.96). As a diagnostic test, the positive predictive value (PPV) was 87% with a negative predictive value (NPV) of 100%. The positive likelihood ratio (+LR) was 10 (95%, CI 3.41-29.2). Conclusions: Our preliminary results demonstrated that TBlue staining of endometrium is a reliable and highly sensitive technique that may be used in the frozen section examination of uterine specimens if a malignancy is suspected. Moreover, TBlue staining of endometrium may prove useful in hysteroscopy, although the technique, interpretation, and indications require further studies. © 2011 Elsevier Ireland Ltd.