Evaluation of a new automated, rapid, colorimetric culture system using solid medium for laboratory diagnosis of tuberculosis and determination of anti-tuberculosis drug susceptibility
International Journal of Tuberculosis and Lung Disease, cilt.8, sa.6, ss.772-777, 2004 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 8 Sayı: 6
- Basım Tarihi: 2004
- Dergi Adı: International Journal of Tuberculosis and Lung Disease
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.772-777
- Anahtar Kelimeler: tuberculosis, Dio-TK Culture System, Lowenstein Jensen medium, Bactec 460 TB
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
SETTING: Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy, Ankara, Turkey, a tertiary referral hospital in a region endemic for tuberculosis. OBJECTIVE: To evaluate the performance of the Dio-TK Culture System (CS), a new, rapid, automated colorimetric culture system. DESIGN: Dio-TK CS results were compared with routinely used Löwenstein Jensen (LJ) medium and Bactec 460 TB. RESULTS: In this study, 449 specimens, mostly sputum samples obtained from 348 patients, were evaluated. Mycobacteria were isolated from 31 (6.9%), 23 (5.1%), 18 (4.0%) and 21 (4.7%) of the specimens using Bactec 12B, LJ, Dio-TK Medium and Dio-TK SLC (selective), respectively. The mean time to detection of growth of 13 isolates by Bactec 12B, Dio-TK Medium, Dio-TK SLC and LJ medium was respectively 8.9, 15.1, 17.0 and 26.1 days. CONCLUSION: Dio-TK may be a practical and rapid culture system for daily use. However, the manufacturer should improve the system to minimise the effects of manipulation errors. Comparative studies with a larger number of isolates are needed to standardise drug concentrations used in anti-tuberculosis drug susceptibility testing.