Effects of papaverine and ascorbic acid on reperfusion induced renal injury: An experimental ischemia and reperfusion model Papaverine ve askorbik asidin reperfüzyona bagli böbrek hasarinda etkileri: Deneysel iskemi ve reperfüzyon modeli
Turkiye Klinikleri Cardiovascular Sciences, cilt.26, sa.2, ss.69-78, 2014 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 26 Sayı: 2
- Basım Tarihi: 2014
- Dergi Adı: Turkiye Klinikleri Cardiovascular Sciences
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.69-78
- Anahtar Kelimeler: Acute kidney injury, Ascorbic acid, Ischemia, Papaverine, Reperfusion
- Sağlık Bilimleri Üniversitesi Adresli: Hayır
Özet
Objective: The reperfusion injury that occurs after the treatment of acute extremity ischemia, is a clinical manifestation causes serious morbidity and mortality. Even if extremity reperfusion is totally provided, it may cause extremity loss, acute renal and respiratory failure. In this experimental study, the effect of vitamin C and papaverine on ischemia-reperfusion injury in kidneys occuring after occlusion-reperfusion of rat infrarenal abdominal aorta was investigated. Material and Methods: 32 Spraque-Dawley female rats were randomly divided into four equal groups (n=8). The same surgical procedure was applied to all rats. The microvascular klamp was put on infrarenal aorta and 60 minutes of ischemia was applied, then the klamp was removed and 120 minutes of reperfusion was supplied. The control and the ischemia-reperfusion groups were not given any drug and the other two groups were given vitamin C and papaverine. The incised renal tissues were fixed with formaldehyde then the samples were stained with Hematoxylin-Eosin. The samples were examined by light microscopy. Results: Meaningful pathological alterations were found out between ischemia and reperfusion group and control group. In vitamin C group, while dilatation of Bowman's capsule, tubular epithelial degeneration, tubular dilatation, and vascular congestion were statistically significant, interstitial inflammatory infiltration was meaningless. In papaverine group, dilatation of Bowman's capsule, degeneration of tubular epithelium, and tubular dilatation were statistically significant, vascular congestion and interstitial inflammatory infiltration were meaningless. Conclusion: We observed that the radical scavenging effect of vitamin C and vasodilatatory effect of papaverine may reduce the renal injury after the reperfusion in the acute lower extremity ischemias by preserving the microcirculation.