Investigation of hemocytes in larvae of Lucilia sericata (Diptera: Calliphoridae)
Biharean Biologist, cilt.14, sa.1, ss.5-9, 2020 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 14 Sayı: 1
- Basım Tarihi: 2020
- Dergi Adı: Biharean Biologist
- Derginin Tarandığı İndeksler: Scopus, BIOSIS, CAB Abstracts, Central & Eastern European Academic Source (CEEAS), Veterinary Science Database
- Sayfa Sayıları: ss.5-9
- Anahtar Kelimeler: Hemocyte, Hemolymph, Innate immunity, Larvae, Lucilia sericata
- Sağlık Bilimleri Üniversitesi Adresli: Hayır
Özet
Larval therapy is used in clinical treatment such as necrotic and infected wounds and larvae of Lucilia sericata are the most commonly used invertebrate for clinical therapy. The purpose of the present study was to investigate characterization of hemocytes in larvae of L. sericata to determine whether there were any differences between larval stages or from other invertebrates in terms of hemocyte types and to provide a better understanding of hemocytes' function. 150 sterile first instar larvae of L. sericata were obtained from Istanbul University, Microbiology and Clinical Microbiology Department in April 2016. Hemolymph of the larvae of L. sericata was collected and hemolymph smears were stained with Wright's stain. In both 1st and 2nd larval stages of L. sericata, three hemocyte types; prohemocytes, plasmatocytes and granulocytes were observed. However, in 3rd larval stage, five distinct types of hemocytes; oenocytoids, adipohemocytes, granulocytes, plasmatocytes and prohemocytes were examined. The types of the hemocytes were characterized by light microscopy according to size, presence or absence of granules and nucleus/cytoplasm ratio.