Phenotypic Colistin Resistance and mcr Genes Presence in Salmonella Serovars Originating from Poultry Farms
Journal of the Hellenic Veterinary Medical Society, cilt.76, sa.3, ss.9491-9498, 2025 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 76 Sayı: 3
- Basım Tarihi: 2025
- Doi Numarası: 10.12681/jhvms.37774
- Dergi Adı: Journal of the Hellenic Veterinary Medical Society
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE
- Sayfa Sayıları: ss.9491-9498
- Anahtar Kelimeler: Antimicrobial resistance, EUCAST, colistin, mcr, Salmonella
- Sağlık Bilimleri Üniversitesi Adresli: Hayır
Özet
The prevalence of antibiotic resistance in bacteria is a matter of concern. Colistin is the last resort for treating infections caused by resistant Enterobacteriaceae. The mcr genes carried and transferred to the plasmid are responsible for the resistance of the bacteria to colistin. The objective of this study was to determine the resistance of Salmonella strains to colistin, isolated from environmental samples taken from poultry farms and serologically identified, and to investigate the presence of mcr genes in resistant strains. A total of 300 Salmonella strains isolated and identified from poultry farms in Turkey between 2014 and 2018 were subjected to phenotypic colistin resistance testing using the microdilution method. The presence of mcr genes was evaluated by multiplex PCR. The antibiotic resistance status of the Salmonella isolates with phenotypic resistance to colistin was analyzed using the Kerby Bauer method. A total of 72 out of 300 Salmonella isolates were phenotypically resistant to colistin. Additionally, resistance to pefloxacin, ampicillin, sulfamethoxasol/trimethoprim, gentamycin, and cefotaxime antibiotics was observed in 34.7%, 5.6%, 4.2%, 2.8%, and 1.4%, respectively. Furthermore, the mcr genes were not detected in the Salmonella strains examined in this study. The results of this study indicate that phenotypic colistin resistance in Salmonella strains isolated from poultry environmental samples is not related to the mcr genes analyzed. The mechanism of resistance may be chromosomal resistance, and the mechanisms should be investigated by whole-genome analysis.