Bacteriological characterization of vancomycin-resistant enterococci in a pediatric hospital in Turkey
Annals of Microbiology, cilt.54, sa.4, ss.543-551, 2004 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 54 Sayı: 4
- Basım Tarihi: 2004
- Dergi Adı: Annals of Microbiology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.543-551
- Anahtar Kelimeler: nosocomial outbreak, VRE, vanA
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Outbreaks with vancomycin-resistant enterococci (VRE) are being reported with increasing frequency in many countries. While seven glycopeptide resistance genotypes have been described in enterococci, vanA and vanB are the most common resistance genotypes. Aims of this study were to detect antibiotic susceptibilities of 25 Enterococcus faecium strains, which caused an outbreak in a child hospital by Etest, to investigate presence of resistance genes of van A, van B or vanC2 type by PCR, and genotype them by PFGE. All isolates conferred high level vancomycin, teicoplanin, erythromycin, and penicillin resistance (MIC>256 mg/L) and were found to be susceptible to ciprofloxacin (MIC≤1 mg/L), tetracycline (MIC≤4 pg/ml) and Q-D (MIC≤1 mg/L). While all of 25 E. faecium isolates were found to be positive for the presence of the vanA genotype, vanB and vanC2 gene products could not be amplified. All of the isolates could be typed by PFGE generating 10 different DNA fragment profiles. As a conclusion, vanA genotype E. faecium strains may cause outbreaks in Turkey. PCR identification of the van genotype of the infecting strains and genotyping them by PFGE give valuable information in outbreak investigation.