Comparison of RT-qPCR and RT-Digital PCR for detection and quantification of BCR-ABL1 transcripts in chronic myeloid leukemia RT-qPCR ve RT-Dijital PCR Yöntemlerinin Kronik Myeloid Lösemi'de BCR-ABL1 Transkriptlerini Tespit Etme ve Ölçümleme Basarilarinin Karsilastirilmasi

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Bahsi T., ERDEM H. B.

Gazi Medical Journal, cilt.30, sa.4A, ss.421-424, 2019 (Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 4A
  • Basım Tarihi: 2019
  • Doi Numarası: 10.12996/gmj.2019.111
  • Dergi Adı: Gazi Medical Journal
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.421-424
  • Anahtar Kelimeler: Chronic myeloid leukemia, BCR-ABL1, RT-qPCR, RT-digital PCR
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • Sağlık Bilimleri Üniversitesi Adresli: Evet

Özet

Aim: Chronic myeloid leukemia (CML) is a hematological malignancy in the group of myeloproliferative neoplasms.Philadelphia chromosome, t(9;22)(q34;q11), results in the BCR/ABL1 fusion gene. The Philadelphia chromosome could be detected in almost all CML cases.RT-qPCR method is still the most commonly used method for monitoring BCR/ABL1 fusion.RTdigital PCR method is an alternative in quantitative measurement of BCR-ABL1 fusion, but there is not enough information in the literature yet. It was planned to evaluate and compare of RT-qPCR and RT-digital PCR for detection and quantification of BCR-ABL1 transcripts in CML. Materials and Methods:Totaly, 39CML patients were performed.Total RNA was extracted with RNA extraction kit (QIAamp RNA Blood Mini Kit). Qiagene Rotor-Gene-Q system was used for RT-qPCR method and QX200TM Droplet DigitalTM PCR (ddPCRTM) system was used for RT-digital PCR testing. Results:There was significant difference between the groups in the BCRABL1/ ABL comparison of the samples (p=0.017) (Table 1). Conclusion:Although the significant difference between RT-digital PCR and RTqPCR in detection and quantification of BCR-ABL1 transcripts in CML, RTdigital PCR is not more sensitive in all samples. Therefore, further research is needed to obtain a clear understanding of the effectiveness of RT-digital PCR.