The sitotoxic effect of gemcitabine on multiple myeloma (RPMI-8226) and Ig G plasma cell leukemia (ARH 77) cell lines Gemsitabinin multipl myelom (RPMI-8226) ve Ig G plazma hücreli lösemi (ARH 77) hücre hatlari üzerindeki sitotoksik etkisi
Gulhane Medical Journal, cilt.54, sa.4, ss.263-266, 2012 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 54 Sayı: 4
- Basım Tarihi: 2012
- Doi Numarası: 10.5455/gulhane.12923
- Dergi Adı: Gulhane Medical Journal
- Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.263-266
- Anahtar Kelimeler: ARH 77, Gemcitabine, Ig G plasma cell leukemia, Multiple myeloma, Nucleoside analog, RPMI-8226
- Sağlık Bilimleri Üniversitesi Adresli: Hayır
Özet
Gemcitabine replaces one of the nucleic acids such as cytidine as a nucleoside analog during DNA replication. The process arrests tumor growth resulting in apoptosis. As a chemotherapeutic agent, gemcitabine has systemic effect on every tumor cells. So, it is used in various carcinomas: non-small cell lung cancer, pancreatic cancer, bladder cancer and breast cancer. Here, multiple myeloma-RPMI-8226 and Ig G plasma cell leukemia-ARH 77 cell lines were treated with gemcitabine in different concentrations. Cytotoxic effect of gemcitabine was detected by XTT assay in cell culture. In RPMI-8226 cell line, lethal effect was detected at the concentration of 400 μmol/L. In ARH-77 cell line, lethal effect was found at the concentration of 200 μmol/L. These results represent us the cytotoxic affect of gemcitabine on multiple myeloma and Ig G plasma cell leukemia tumor cells. It is needed to do further experiments for finding efficiency of multiple myeloma and plasma cell leukemia treatment with gemcitabine. © Gülhane Askeri Tip Akademisi 2012.