Simple Determination of Cystine in Artificial Urine and Drug Matrices Using Digital Image Colorimetry
Analytical Letters, cilt.59, sa.5, ss.920-934, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 59 Sayı: 5
- Basım Tarihi: 2026
- Doi Numarası: 10.1080/00032719.2025.2517118
- Dergi Adı: Analytical Letters
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, Chimica, Academic Search Ultimate (EBSCO), Biomedical Reference Collection: Corporate Edition (EBSCO)
- Sayfa Sayıları: ss.920-934
- Anahtar Kelimeler: Cystine, determination, digital image colorimetry, smartphone, spectrophotometry, urine
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Smartphone-based digital image colorimetry was developed for the determination of cystine in pharmaceutical formulations and artificial urine samples. A lab-made colorimetric box was designed for capturing the digital images of sample solutions using a smartphone. The developed method employs a simple colorimetric method called nitroprusside test which specifically targets free -SH groups. Cystine was reduced to cysteine before the colorimetric test was applied. The color intensity is inversely related to the concentration of the analyte. The best linearity was obtained with the G color channel. Digital image processing was performed using the RGB Color Detector mobile application to obtain analytical data. The developed method exhibited good linearity in the range of 29.7–00.3 mg/kg. The limits of detection and quantification were 9.7 mg/kg and 32.3 mg/kg, respectively. The percent recoveries were 104.2 ± 7.8% and 108.7 ± 4.6%, which proved the accuracy and applicability of the developed method.