Maturation outcomes are improved following Cryoleaf vitrification of immature human oocytes when compared to choline-based slow-freezing
Journal of Assisted Reproduction and Genetics, cilt.28, sa.12, ss.1183-1192, 2011 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 28 Sayı: 12
- Basım Tarihi: 2011
- Doi Numarası: 10.1007/s10815-011-9674-x
- Dergi Adı: Journal of Assisted Reproduction and Genetics
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1183-1192
- Anahtar Kelimeler: Immature oocyte, Choline-based slow-freezing, Cryoleaf vitrification, Spindle, Chromosomes
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Purpose: The cryopreservation of immature oocytes permits oocyte banking for patients at risk of losing their fertility. However, the optimum protocol for such fertility preservation remains uncertain. Methods: The present study investigated the survival, maturation, cytoskeletal and chromosome organization of sibling immature oocytes leftover from controlled ovarian stimulation cycles, that were either slow-frozen (with choline-substitution) or vitrified. A comparison group included oocytes that were never cryopreserved. Results: Among the three groups, comparable rates were observed for both survival (67-70%) and polar body extrusion (59-79%). Significantly more oocytes underwent spontaneous activation after IVM following slow-freezing compared with either vitrification or no cryopreservation. Likewise, the incidence of spindle abnormalities was greatest in the slow-frozen group, with no differences in spindle morphometrics or chromosome organization. Conclusions: While the overall incidence of mature oocytes with normal bipolar spindles from warmed immature oocytes was low, the yield using Cryoleaf vitrification was slightly superior to choline-based slow-freezing. © 2011 Springer Science+Business Media, LLC.