Decellularization of trachea with combined techniques for tissue-engineered trachea transplantation
Clinical and Experimental Otorhinolaryngology, cilt.12, sa.1, ss.86-94, 2019 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 12 Sayı: 1
- Basım Tarihi: 2019
- Doi Numarası: 10.21053/ceo.2018.00486
- Dergi Adı: Clinical and Experimental Otorhinolaryngology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.86-94
- Anahtar Kelimeler: Trachea, Scaffold, Freeze Drying, Tissue Engineering, Deoxycholic Acid
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objectives. The purpose of this study is to shorten the decellularization time of trachea by using combination of physical, chemical, and enzymatic techniques. Methods. Approximately 3.5-cm-long tracheal segments from 42 New Zealand rabbits (3.5±0.5 kg) were separated into seven groups according to decellularization protocols. After decellularization, cellular regions, matrix and strength and endurance of the scaffold were followed up. Results. DNA content in all groups was measured under 50 ng/mg and there was no significant difference for the glycos-aminoglycan content between group 3 (lyophilization+deoxycholic acid+de-oxyribonuclease method) and control group (P=0.46). None of the decellularized groups was different than the normal trachea in tensile stress values (P>0.05). Glucose consumption and lactic acid levels measured from supernatants of all decellularized groups were close to group with cells only (76 mg/dL and 53 mg/L). Conclusion. Using combination methods may reduce exposure to chemicals, prevent the excessive influence of the matrix, and shorten the decellularization time.