Serum levels of indoleamine 2,3 dioxygenase in erectile-dysfunction patients Erektil disfonksiyonlu hastalarda serum indolamin 2,3 dioksijenaz düzeyi
Journal of Clinical and Analytical Medicine, cilt.8, sa.6, ss.449-452, 2017 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 8 Sayı: 6
- Basım Tarihi: 2017
- Doi Numarası: 10.4328/jcam.5082
- Dergi Adı: Journal of Clinical and Analytical Medicine
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.449-452
- Anahtar Kelimeler: Oxidative Stress, Erectile Dysfunction, Indoleamine 2,3-Dioxygenase
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Aim: Indoleamine 2,3-dioxygenase (IDO) is a widely expressed enzyme that cleaves the pyrrole ring from tryptophan by using superoxide anion radicals (O2). Tryptophan metabolite kynurenines have been shown to accelerate apoptosis in endothelial cells. Given that oxidative stress and endothelial dysfunction has been implicated in a pathologic role that results in erectile dysfunction. We aimed here to test the hypothesis that IDO is involved in erectile dysfunction and that the serum IDO level is related to the serum total antioxidant capacity (TAC). Material and Method: Twenty-three erectile dysfunction patients, aged 48±5 years, and 20 age-matched (46±5 years) male controls were included. The total antioxidant capacity (TAC) was assayed in terms of the ferric-reducing ability of plasma (FRAP). Serum IDO levels were determined in duplicate using a commercial double-antibody sandwich technique enzyme-linked immunosorbent assay (ELISA) kit from Sunred Biotechnology Company (Shanghai, China). Results: Serum IDO levels were similar in ED patients 25.7(IQR=37.4) ng/ml compared to the control group 28.0(IQR=63.0) ng/ml (p=0.064). TAC levels were not different in the ED patients 833±189 compared to the control group 921±191μmol/L (p=0.084). Our analysis indicated that IDO level was not correlated with the International Index of Erectile Function (IIEF-5) score (r =0.281, p = 0.067). Discussion: These data suggest that changes in IDO expression are not involved in erectile dysfunction in humans.