Serum perilipin-2 levels in a rat model of liver ischemia-reperfusion injury
Biotechnic and Histochemistry, cilt.100, sa.6, ss.360-370, 2025 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 100 Sayı: 6
- Basım Tarihi: 2025
- Doi Numarası: 10.1080/10520295.2025.2528036
- Dergi Adı: Biotechnic and Histochemistry
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, MEDLINE
- Sayfa Sayıları: ss.360-370
- Anahtar Kelimeler: Acute liver injury, biomarker, ischemia reperfusion, liver damage, perilipin-2
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Liver ischemia and ischemia/reperfusion (I/R) injury are common in hepatic resection, trauma surgery, and transplantation and contribute to postoperative morbidity and mortality. This study aimed to investigate the relationship between early histopathological changes due to liver I/R injury and serum levels of perilipin-2 (Plin2) and other oxidative stress biomarkers. Fifty female Wistar albino rats were divided into five groups: Group 1 (control), Group 2 (ischemia for 30 minutes), and Groups 3–5 (ischemia followed by 1, 2, or 3 hours of reperfusion). Intracardiac and arterial blood samples were collected for biochemical analysis, while liver tissue samples were used for histopathological examination. Serum levels of Plin2, ischemia-modified albumin (IMA), total antioxidant status (TAS), and total oxidant status (TOS) were measured. Plin2 levels were significantly lower in the ischemia group compared to others (p < 0.01). The control group had significantly lower IMA, TOS, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) levels (p < 0.01) and lower TAS levels (p < 0.05). Rats with Grade 1 liver injury showed significantly lower Plin2 (p < 0.01) and higher IMA levels (p < 0.05). Reduced serum Plin2 following ischemia suggests its potential as a biomarker for acute liver injury. Further studies are needed to clarify its role across different reperfusion durations.