Investigation of isolated anti-HBc positivity and occult HBV infection in HBsAg negative donated blood HBsAg negatif bağış kanlarında izole anti-HBc pozitifliği ve okült HBV enfeksiyonu araştırılması
Turk Hijyen ve Deneysel Biyoloji Dergisi, cilt.82, sa.1, ss.33-40, 2025 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 82 Sayı: 1
- Basım Tarihi: 2025
- Doi Numarası: 10.5505/turkhijyen.2025.57701
- Dergi Adı: Turk Hijyen ve Deneysel Biyoloji Dergisi
- Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.33-40
- Anahtar Kelimeler: Blood donor, HBV DNA PCR, HBV DNA PCR, Kan donörü, occult HBV infection, okült HBV enfeksiyonu
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objective: Occult HBV infection (OHI) can be defined as the presence of HBV DNA in the liver tissue of HBsAg negative individuals (with detectable or undetectable HBV DNA in the serum) by current sensitive tests, except window period of infection. This study was carried out to investigate isolated anti-HBc positivity and OHI, by using anti-HBc, HBV DNA PCR and anti-HBs, in the plasma samples of HBsAg negative donated blood. Methods: The one thousand and sixty three plasma samples of HBsAg, anti-HCV and anti-HIV negative blood which were donated between 19 March and 28 April 2010 stored at -80 ºC for one year in accordane with National Blood and Blood Products Guide, were included in the study. OHB was investigated by using anti-HBc and HBV DNA PCR in all plasma samples which were included in the study. In anti-HBc and/or HBV DNA PCR positive samples, anti-HBs investigation was conducted. Results: Anti-HBc positivity was detected in 87 (8.2%) donor plasma samples. The mean age of antiHBc positive donors or isolated anti-HBc positive donors or anti-HBc/anti-HBs positive donors were; 30±11.5 years, 38±15.9 years and 28.8±10.4 years, respectively. Among all plasma samples included in the study; the rate of isolated anti-HBc positivity was 1.03% (n=11) and isolated anti-HBc positivity was seen significantly in older age, compared to anti-HBc positivity with anti-HBs positivity (p=0.047). As a result of this study, HBV DNA PCR positivity was not detected in any of the donor plasma samples. Conclusion: The rates of isolated anti-HBc positivity in blood donors admitted to our center was found lower than earlier studies conducted among blood donors in Turkey. In Turkey, although there is no legal obligation, Kızılay Regional Blood Centers have started to work on NAT in onated blood as of 1 November 2014. We suggest that large-scale studies to investigate the use of antiHBc and/or nucleic acid amplification tests in addition to HBsAg for screening of blood donors.