Proliferating cell nuclear antigen and its prognostic significance in childhood Hodgkin's disease: Preliminary study
Turkish Journal of Cancer, cilt.28, sa.2, ss.59-67, 1998 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 28 Sayı: 2
- Basım Tarihi: 1998
- Dergi Adı: Turkish Journal of Cancer
- Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.59-67
- Anahtar Kelimeler: Cellular proliferation, Hodgkin's disease, Immunohistochemistry, Prognosis, Proliferating cell nuclear antigen
- Sağlık Bilimleri Üniversitesi Adresli: Hayır
Özet
To evaluate the distribution of proliferating cell nuclear antigen and its prognostic importance, 27 cases of pediatric Hodgkin's disease were studied. The monoclonal antibody PC10 picks up the proliferating cell nuclear antigen associated With the cell cycle and assessed cellular proliferation status. In our study, to detect the monoclonal antibody in formalin fixed, paraffin embedded tissues the enhanced polymerase one step technique was applied. Nuclear and/or cytoplasmic proliferating cell nuclear antigen immunoreactivity was observed in Reed-Sternberg cells and variants and also in activated lymphocytes. Proliferating cell nuclear antigen immunoreactive cells, Reed-Sternberg cells and variants were counted as cell/mm2. Eighty four per cent of Reed-Sternberg cells and variants were found to be anti- proliferating cell nuclear antigen positive, and mean anti-proliferating cell nuclear antigen positive cell count was found as 15.4 cell/mm2. Overall survival was found significantly shorter in patients with high anti- proliferating cell nuclear antigen(+) cell count (p=0.02). Our results suggest that proliferating cell nuclear antigen is a valid marker of proliferation; the cells of reactive tissue are also proliferating and they are as important as malign cells; the assessment of cellular proliferation determined by using proliferating cell nuclear antigen, can be used as a prognostic factor in childhood Hodgkin's disease.