Stenotrophomonas raiganjensis sp. nov., an extensively drug-resistant bacterium isolated from Bombyx mori L., described under the SeqCode
Systematic and Applied Microbiology, cilt.49, sa.2, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 49 Sayı: 2
- Basım Tarihi: 2026
- Doi Numarası: 10.1016/j.syapm.2026.126699
- Dergi Adı: Systematic and Applied Microbiology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, EMBASE, MEDLINE, Natural Science Collection (ProQuest), Biological Science Database (ProQuest), Earth, Atmospheric, & Aquatic Science Collection (ProQuest), Health Research Premium Collection (ProQuest)
- Anahtar Kelimeler: SeqCode, AMR, XDR, Stenotrophomonas, MLSA, Pangenome analysis, ANI value, dDDH value
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
SeqCode allows a single strain or a high-quality genome to serve as type material, making it compatible with modern genomics and biodiversity governance frameworks, especially for countries such as India. We, therefore, describe a novel bacterium, designated RAC2TS under the SeqCode. RAC2TS was isolated from the hemolymph of infected Bombyx mori L. and characterized using a polyphasic taxonomic approach. While 16S rRNA gene sequence based phylogenetics showed Stenotrophomonas maltophilia NBRC 14161ᵀ to be the closest relative, multilocus sequence analysis (MLSA) based on seven housekeeping genes ( atpD , gapA , guaA , mutM , nuoD , ppsA , recA ) and genome-based relatedness indices confirmed RAC2TS as a distinct lineage within the genus Stenotrophomonas . Whole-genome analysis identified a 4.6 Mbp genome with 66.5% G + C content; and 4276 coding sequences, including several antimicrobial resistance genes alongside multidrug efflux pump sme operon genes and a single virulence-associated gene, pilG . RAC2TS showed 91–93% average nucleotide identity (ANI) and 41–48% digital DNA-DNA hybridization (dDDH) with closely related taxa. Pangenome analysis revealed an open pangenome (α = 0.257), with 12,623 gene clusters including 229 core genes and 6994 strain-specific genes. Antibiotic susceptibility testing demonstrated extensive drug resistance (XDR) characteristics of RAC2TS. Fatty acid methyl ester (FAME) analysis revealed isoC15:0 (24.56%), anteiso-C15:0 (14.27%), and summed feature 3 (16:1 ω7c/16:1 ω6c; 11.18%) as major components. Combined phenotypic, chemotaxonomic, phylogenomic, and genomic evidence supports the recognition of RAC2TS as a novel species within the genus Stenotrophomonas . Under the SeqCode, we propose the name S. raiganjensis sp. nov. for strain RAC2TS (=MCM-B-1506TS) with the type genome GCA_030658505.2TS.