Effects of intra-abdominal pressure increase on intestinal ischemia and bacterial translocation in experimental sepsis model
Saudi Medical Journal, cilt.32, sa.8, ss.813-817, 2011 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 32 Sayı: 8
- Basım Tarihi: 2011
- Dergi Adı: Saudi Medical Journal
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.813-817
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objectives: To investigate the safety of laparoscopic intervention for diagnosis and treatment at 8 mm Hg pressure in one-hour period on acute peritonitis related intra-abdominal sepsis model. Methods: In this study, we included 32 female Wistar-Albino rats, weighing 250 ± 20 g, and divided them into 4 groups. This study was conducted in Istanbul University Experimental Medical Research Institution (DETAE) laboratory from April to May 2009. Intraabdominal sepsis was created with intraperitoneal (i.p.) one mL (10 9 CFU/mL) Escherichia coli (E. coli) injection, and pneumoperitoneum was formed with CO 2 insufflation at 8 mm Hg pressure for one hour i.p. The rats were administered with: Group 1-one mL i.p. isotonic saline; Group 2-one mL i.p. isotonic saline + pneumoperitoneum; Group 3-i.p E. coli; and Group 4-i.p. E.coli + pneumoperitoneum. Data were analyzed using the Statistical Package for Social Sciences version 15 for Windows (SPSS Inc, Chicago, IL, USA). Results: Fever and leukocyte values were considered high in Groups 3 and 4 compared with Groups 1 and 2 (p=0.001). The administered reproduction ratio of the E. coli strain was determined as 0% in Groups 1 and 2, and 100% in Groups 3 and 4. Conclusion: In this study, as pneumoperitoneum was formed for one hour at 8 mm Hg pressure, in case of intra-abdominal derived sepsis where emergency intervention is needed, we consider that laparoscopic approaches with low pressure may be used safely for diagnosis and treatment.