Cytogenetic abnormalities in acute leukemia patients: Results of conventional cytogenetics and fluorescent in situ hybridization analyses
Balkan Journal of Medical Genetics, cilt.8, sa.1-2, ss.33-38, 2005 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 8 Sayı: 1-2
- Basım Tarihi: 2005
- Dergi Adı: Balkan Journal of Medical Genetics
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.33-38
- Anahtar Kelimeler: Acute lymphoblastic leukemia (ALL), Acute myeloblastic leukemia (AML), Inversion 16, MLL, t(15;17), t(9;22)
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Acute leukemia is classified as acute lymphoblastic leukemia (ALL) or acute myeloblastic leukemia (AML), according to the type of the progenitor cell from which the disease originates Chromosome rearrangements play a crucial role in the pathogenesis, and conventional cytogenetics has a primary importance in diagnosis of patients. More sensitive methods, such as fluorescent in situ hybridization (FISH), are recommended to reveal cryptic rearrangements or when karyotype analysis is not possible because of poor chromosome quality. In this study, we compared the results of conventional cytogenetics and FISH in 71 acute leukemia patients. In 28 patients, we detected chromosome rearrangements either by conventional cytogenetics or by FISH. In 15 patients, conventional cytogenetics revealed a normal karyotype, whereas FISH revealed the rearrangements. In two patients, conventional cytogenetics results were not available. In these cases, FISH enabled detection of t(9;22). As a result, we concluded that both methods need to be performed at initial diagnosis, so as to guide the treatment and follow-up of patients.