Extracellular 70-kd heat shock protein in mid-trimester amniotic fluid and its effect on cytokine production by ex vivo-cultured amniotic fluid cells
American Journal of Obstetrics and Gynecology, cilt.194, sa.3, ss.694-698, 2006 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Özet
- Cilt numarası: 194 Sayı: 3
- Basım Tarihi: 2006
- Doi Numarası: 10.1016/j.ajog.2006.01.066
- Dergi Adı: American Journal of Obstetrics and Gynecology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.694-698
- Anahtar Kelimeler: 70-kd heat shock protein, amniotic fluid, ex vivo culture, tumor necrosis factor-alpha, toll-like receptor, Mycoplasma hominis
- Sağlık Bilimleri Üniversitesi Adresli: Hayır
Özet
Objective: The 70-kd heat shock protein is released from cells in response to stress and functions as a regulator of innate immunity. We hypothesized that 70-kd heat shock protein in mid-trimester amniotic fluid might regulate local immune system activation. Study design: Amniotic fluid that was obtained from 200 women who underwent amniocentesis at 15 to 19 weeks of gestation was tested by enzyme-linked immunosorbent assay for 70-kd heat shock protein, tumor necrosis factor-α, and interleukin-1β and -6. The amniotic fluid cellular fraction also was evaluated for Mycoplasma hominis by gene amplification. Whole amniotic fluids were incubated ex vivo in medium alone or medium that contained peptidoglycan, a TLR2 ligand, or lipopolysaccharide, a TLR4 ligand. After 24 hours, the supernatants were collected and assayed for 70-kd heat shock protein. The influence of exogenous 70-kd heat shock protein on tumor necrosis factor-α and interleukin-1β and -6 production by whole amniotic fluid was assessed similarly. Results: The 70-kd heat shock protein was detected in all amniotic fluids with a median (range) of 11.5 ng/mL (1.2-76.7). The intra-amniotic 70-kd heat shock protein concentration was correlated positively only with amniotic fluid tumor necrosis factor-α levels (P = .0002). Detection of M hominis was associated with an increased 70-kd heat shock protein concentration (median, 17.2 ng/mL; P = .01). The addition of peptidoglycan resulted in a stimulation of 70-kd heat shock protein production, and exogenous 70-kd heat shock protein stimulated the release of tumor necrosis factor-α by amniotic fluid cells. Conclusion: The 70-kd heat shock protein is released from cells in mid-trimester amniotic fluid as a consequence of TLR2 stimulation and potentiates tumor necrosis factor-α production. © 2006 Mosby, Inc. All rights reserved.