Analysis of chromosomal aberrations and FLT3 gene mutations in childhood acute myelogenous leukemia patients Çocukluk Çaǧı Akut Myeloid Lösemi Hastalarında Kromozomal Deǧişiklikler ve FLT3 Geni Mutasyonlarının Araştırılması
Turkish Journal of Hematology, cilt.29, sa.3, ss.225-232, 2012 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 29 Sayı: 3
- Basım Tarihi: 2012
- Doi Numarası: 10.5505/tjh.2012.24392
- Dergi Adı: Turkish Journal of Hematology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.225-232
- Anahtar Kelimeler: Childhood AML, FLT3 gene mutations, ITD, D835 mutations, Chromosomal translocations
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objective: To identify the well-known common translocations and FLT3 mutations in childhood acute myelogenous leukemia (AML) patients in Turkey. Material and Methods: The study included 50 newly diagnosed patients in which t(15;17), t(8;21), and inv(16) chromosomal translocations were identified using real-time PCR and FLT3 gene mutations were identified via direct PCR amplification PCR-RE analysis. Results: In all, t(15;17) chromosomal aberrations were observed in 4 patients (8.0%), t(8;21) chromosomal aberrations were observed in 12 patients (24.0%), inv(16) chromosomal aberrations were observed in 3 patients (6.0%), and FLT3- ITD mutations were observed in 2 patients (4.0%); FLT3-D835 point mutation heterozygosity was observed in only 1 patient (2.0%) patient. Conclusion: Despite of the known literature, a patient with FLT3-ITD and FLT3-D835 double mutation shows a better survival and this might be due to the complementation effect of the t(15;17) translocation. The reportedmutation rate in this article (4%) of FLT3 gene seems to be one of the first results for Turkish population.