Investigation of compatibility of severe acute respiratory syndrome coronavirus 2 reverse transcriptase-PCR kits containing different gene targets during coronavirus disease 2019 pandemic
Future Virology, cilt.15, sa.8, ss.515-524, 2020 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 15 Sayı: 8
- Basım Tarihi: 2020
- Doi Numarası: 10.2217/fvl-2020-0169
- Dergi Adı: Future Virology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, EMBASE
- Sayfa Sayıları: ss.515-524
- Anahtar Kelimeler: coronavirus, COVID-19, RT-PCR, SARS-CoV-2
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Aim: In the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), reverse transcriptase-PCR (RT-PCR) technique is often used. We evaluated the compatibility of SARS-CoV-2 RT-PCR kits containing different gene targets during the pandemic. Materials & methods: Samples were tested by Bio-Speddy® (RdRp gene) and Diagnovital® (RdRp + E genes). The correlation between two assays were determined by Deming regression analysis and chi-square analyses. Results: Diagnovital PCR kit showed amplification in a narrow Ct range and conveniently sharper exponential amplification curves than Bio-Speedy PCR kit. While the correlation between the findings of the two kits was apparent even with single gene target, this correlation increased when a secondary biomarker was added to the correlation calculations. Conclusion: We have observed high correlation between different PCR kits, however, using different PCR kits during the pandemic may provide a more accurate diagnosis of SARS-CoV-2, since despite correlation there are a number of patients showing contradicting diagnosis.