Evaluation of the Relationship between Antioxidant Gene Polymorphisms (Endothelial Nitric Oxide Synthase, Myeloperoxidase, Uncoupling Protein 2) and Breast Cancer
Eurasian Journal of Medicine and Oncology, cilt.4, sa.4, ss.265-270, 2020 (ESCI, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 4 Sayı: 4
- Basım Tarihi: 2020
- Doi Numarası: 10.14744/ejmo.2020.99886
- Dergi Adı: Eurasian Journal of Medicine and Oncology
- Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.265-270
- Anahtar Kelimeler: Breast cancer, endothelial nitric oxide synthase, myeloperoxidase, uncoupling protein 2
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objectives: This study aimed to determine the relationship of breast cancer development with the polymorphisms of endothelial nitric oxide synthase (eNOS), myeloperoxidase (MPO), and uncoupling protein 2 (UCP2) genes. Methods: The study included 60 breast cancer patients and 70 healthy controls. After exclusion criteria, 37 patients and 70 healthy controls were enrolled into study. The functional variants studied were intron-4 variable number of tandem repeats (VNTR),-G463A, and-866G/A variants for the eNOS, MPO, and UCP-2 genes, respectively. The polymerase chain reaction (PCR) and/or PCR-restriction fragment length polymorphism (RFLP) methods were used for genotyping. The distribution of genotype frequencies of the eNOS, MPO, and UCP2 genes were compared between the breast cancer patients and healthy controls using the Chi-square test. Results: The BB genotype of the eNOS gene variant (intron-4 VNTR) was associated with a significantly decreased risk of breast cancer (OR=0.56; 95%CI, 0.463–0.676; p=0.001); the AA and AB genotypes were not associated with the risk of breast cancer as reported in our previous work. No significant association was determined between the risk of breast cancer and any genotype of the MPO gene variant. While the AA (OR=8.167; 95% CI, 2.785–23.951; p=0.001) and AG (OR=4.341; 95% CI, 1.679–11.222; p=0.002) genotypes of the UCP2 gene variant were associated with significantly decreased risk of breast cancer, GG genotype of the UCP2 gene variant was associated with significantly increased risk (OR=5.0; 95% CI, 2.207–11.327; p=0.001). Conclusion: Outcomes of this study revealed that breast cancer was associated with BB genotype of the intron-4 VNTR variant of the eNOS gene and AA, AG, and GG genotypes of the-866G/A variant of the UCP2.