Examination of Cognitive Functions and Hippocampal Synaptophysin Levels in an Experimental Schizophrenia Model Deneysel Şizofreni Modelinde Bilişsel Fonksiyonlar ve Hipokampal Sinaptofizin Düzeylerinin İncelenmesi
Journal of Uludag University Medical Faculty, cilt.49, sa.3, ss.367-373, 2023 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 49 Sayı: 3
- Basım Tarihi: 2023
- Doi Numarası: 10.32708/uutfd.1381823
- Dergi Adı: Journal of Uludag University Medical Faculty
- Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.367-373
- Anahtar Kelimeler: Endophenotype, Memory, Prepulse Inhibition, Schizophrenia, Synaptophysin
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Schizophrenia is a chronic brain disease and is clinically characterized by positive, negative and cognitive symptoms. Cognitive symptoms can be observed starting from the prodromal period of the disease. The aim of this study is to investigate the cognitive functions of rats and hippocampal levels of the presynaptic protein synaptophysin in an experimental schizophrenia model created by prepulse-mediated inhibition (PPI) of the startle reflex. In the study, 30 male Wistar type rats were subjected to basal PPI measurements and were ranked from low to high according to these values. After the first 10 rats were divided into the "low" and the last 10 rats into the "high" inhibition group, they were subjected to the Morris Water Maze (MWM) test for 5 days. At the end of the test, the rats were sacrificed, hippocampus regions were excised, and the presynaptic synaptophysin protein was analyzed by Western Blot method. According to the results, while there was no difference between the learning levels of both groups, the frequency of memory functions frequency to cross the platform area (p <0.05) and time spent in the platform area (p <0.05) parameters were found to be significantly lower in rats in the low PPI group. Synaptophysin levels were similarly found to be significantly (p<0.01) lower in rats in the low PPI group. The results of our study show that some cognitive functions and presynaptic protein synaptophysin levels are significantly lower in rats with low PPI values compared to those with high PPI values. These results also supported the importance of the PPI test, which has been used as a reliable method in schizophrenia research for a long time, by emphasizing its comparable outcomes in human and animal studies of schizophrenia.