Evaluation of FASTPlaquetb-RIF™ for determination of rifampicin resistance in mycobacterium tuberculosis complex isolates
International Journal of Tuberculosis and Lung Disease, cilt.7, sa.3, ss.284-288, 2003 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 7 Sayı: 3
- Basım Tarihi: 2003
- Dergi Adı: International Journal of Tuberculosis and Lung Disease
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.284-288
- Anahtar Kelimeler: Mycobacteriophage, Rifampicin resistance, Susceptibility test, Tuberculosis
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
SETTING: The Microbiology and Clinical Microbiology Department, Gulhane Military Medical Academy, Ankara, Turkey, a tertiary referral hospital in a region endemic for tuberculosis. OBJECTIVE: To evaluate rifampicin resistance of Mycobacterium tuberculosis complex strains using FASTPlaqueTB-RIF™, a rapid and novel bacteriophage-based susceptibility technique. DESIGN: Results of isolates tested with the BACTEC 460 TB system were compared with FASTPlaqueTB-RIF™. RESULTS: Susceptibility to rifampicin of M. tuberculosis complex isolates was tested for 88 isolates using FASTPlaqueTB-RIF™. Sixty-seven isolates were susceptible and 21 were resistant to rifampicin using the BACTEC 460 TB system. Overall accuracy for FASTPlaqueTB-RIF™ was 94.3% (95%-CI% 87.3-97.5) for the detection of rifampicin susceptibility. The sensitivity and specificity of FASTPlaqueTB-RIF™ were respectively 100.0% (95%CI 84.5-100) and 92.5% (95%CI 83.6-96.7). CONCLUSION: This study demonstrates that FASTPlaqueTB-RIF™ is a rapid and inexpensive test which has a good correlation with the BACTEC 460 TB system.