Effects of diethyldithiocarbamate on diabetogenic action of alloxan in Guinea-pigs: An ultrastructural study
Acta Pharmaceutica Turcica, cilt.39, sa.2, ss.65-71, 1997 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 39 Sayı: 2
- Basım Tarihi: 1997
- Dergi Adı: Acta Pharmaceutica Turcica
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.65-71
- Anahtar Kelimeler: Alloxan, Diabetes, Diethyldithiocarbamate, Superoxide dismutase
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
In attempts to induce diabetes in guinea-pigs by alloxan, a diabetes- like state appears after injection, but this situation is followed by islet β-cell regeneration and insulin insufficiency disappears within two weeks. In order to test whether diethyldithiocarbamate (DDC), a superoxide dismutase (SOD) inhibitor, enhances diabetogenic action of alloxan in guinea-pigs, we divided guinea-pigs into three groups, as follows: (i) saline plus saline treated controls. (ii) saline plus alloxan treated guinea-pigs (ALL) and (iii) DDC plus alloxan-treated guinea-pigs (DDC+ALL). After an overnight fast, DDC (750 mg/kg) or saline were injected intraperitoneally to the guinea-pigs. After 2.5 hours, alloxan (200 mg/kg) or saline were injected to the guinea-pigs by intracardiac route, 7 and 14 days after injections, the guinea-pigs were weighted, fasting serum glucose and oral glucose tolerance testing (OGTT) were performed. After sacrification, light and electron microscobic investigations of pancreas were done. Alloxan stopped weight gain and increased glucose levels slightly 7 and 14 days after the injection. Additional DDC administration decreased the weight gain and increased the glucose levels further but not significantly, 7 days after the injection, while alloxan increased the OGTT index slightly, DDC addition significantly increased the index, 14 days after the injections, we observed no significant effect of the treatments on the OGTT index. Our light and electron microscopic findings were in parallel with our biochemical findings. The islets of Langerhans were smaller in ALL and DDC+ALL groups than those in the control group. α and δ cells at the islets remained unaltered, while β- cells decreased in number. At the 14th day, the islets of the ALL and DCC+ALL treated guinea-pigs appeared almost normal. These results suggest that, guinea-pigs are not susceptible to diabetogenic action of alloxan, even if cellular SOD is inhibited. So, the mechanism of this resistance may depend on other enzimatic pathways which differs between guinea-pigs and other alloxan- susceptible species.