Genomic damage in patients with chronic renal failure
Turkiye Klinikleri Journal of Medical Sciences, cilt.29, sa.6, ss.1392-1397, 2009 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 29 Sayı: 6
- Basım Tarihi: 2009
- Dergi Adı: Turkiye Klinikleri Journal of Medical Sciences
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1392-1397
- Anahtar Kelimeler: Micronucleus, chromosome-defective, sister chromatid exchange, renal failure
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objective: Chronic renal failure (CRF) is associated with a high incidence of cancer. The frequencies of sister chromatid exchange (SCE) and micronucleus (MN) in peripheral blood lymphocytes (PBL) is extensively used as biomarkers of chromosomal damage and genome stability in human populations. The aim of this study was to determine the SCE and MN rates of the PBL in patients with chronic renal failure (CRF). Material and Methods: This study was conducted between February 2005 and June 2006 in the Erzurum State Hospital. We analyzed lymphocytes from 34 (12 females and 22 males) CRF patients and 30 (14 females and 16 males) normal controls for SCE and MN frequencies. In the patient group, the mean dialytic age was 3.23 ± 1.56 years (range 1 to 6 years). Results: SCE rate was significantly increased in CRF patients (9.48 ± 1.23) compared with controls (4.85 ± 0.81) (p< 0.001). Similarly, MN incidence was significantly higher in CRF patients (25.82 ± 7.03) compared with controls (12.79 ± 4.62) (p< 0.001). The SCE and MN rates positively correlated with creatinin levels (r= 0.906; p< 0.001 and r= 0.534; p<0.01, respectively). However, the SCE and the MN rates did not correlate with the blood levels of urea (r= 0.427, p> 0.05 and r= 0.409, p> 0.05, respectively). Conclusion: Our results suggest that high SCE and MN frequencies reflect increased DNA damage in CRF patients and this may contribute to the increased incidence of many chronic diseases in such patients. Copyright © 2009 by Türkiye Klinikleri.