Istanbul'un sivrisinek faunasi ve Culex pipiens larvalarinin Bacillus cinsi bakterilere karsi duyarliligi I˙stanbul'un sivrisinek faunasi ve Culex pipiens l arvalarinin Bacillus cinsi bakterilere karşi duyarliliği
Turk Hijyen ve Deneysel Biyoloji Dergisi, cilt.73, sa.2, ss.149-156, 2016 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 73 Sayı: 2
- Basım Tarihi: 2016
- Doi Numarası: 10.5505/turkhijyen.2016.48254
- Dergi Adı: Turk Hijyen ve Deneysel Biyoloji Dergisi
- Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.149-156
- Anahtar Kelimeler: Bacillus sphaericus, Bacillus sphaericus - Bacillus thuringiensis israelensis, Bacillus thuringiensis, Culex pipiens, Unat's fish broth
- Sağlık Bilimleri Üniversitesi Adresli: Hayır
Özet
Objective: In the study the impact of the Bacillus species bacteria used in the fight against larvae of Culex pipiens which constitute mosquito fauna of Istanbul was evaluated. Methods: In the study, 501 samples were taken from the sources of mosquito larvae before disinfection, in 39 towns of Istanbul, from 15 April to the end of June, in 2013. Samples were brought to the laboratory, by maintaining at 24 °C to ensure larvae come to stages III and IV, and some of them to adult mosquitoes. Lyophilized preparations of Bacillus sphaericus (200 g/ha, 300 g/ha and 400 g/ ha); Bacillus thuringiensis H-14 (200 g/ha, 300 g/ha and 400 g/ha); Bacillus sphaericus (H5a5b, Strain 2362) + Bacillus thuringiensis israelensis (H-14, Strain AM65-52) (5000 g/ha) species which are used as larvacide by Istanbul Metropolitan Municipality agaist C. pipiens III and IV stage larvae, were used. The cultures (6,1x108 CFU/ml and 12,6x108 CFU/ml) which prepared from these lyophilized preparations in the medium Unat's fish broth were used. Remaining mosquito larvae and adult mosquitoes were examined under stereo and light microscope to identify the species of mosquitoes. Results: The larvae of C. pipiens species were observed in all samples taken from 39 towns of Istanbul. In samples from Buyukcekmece, Silivri and Umraniye districts, beside densely presented C. pipiens larvae, a small amount of Anopheline and Aedes species larvae were also observed. The first larval mortality began in 50th minutes in the experiment with lyophilized preparations and cultures prepared in Unat's fish broth (12,6x108 CFU/ml density). In the experiments used culture concentration of 6.1x108 CFU/ml, the first larvae mortality was observed in 60th minute. Conclusion: In all experiments, it was observed that all of the larvae died in 6th hours. Cultures prepared in Unat's fish broth were determined to be more effective than lyophilized preparations. It was determined that the reduced density of cultures caused a delay in starting time of mortality but overall time for all larvae deaths was unchanged.