Effect of Different Propofol Concentrations on Rat Lung Mesenchymal Stem Cells Propofolün Farklı Konsantrasyonlarının Rat Akciğer Mezenkimal Kök Hücreler Üzerindeki Etkisi
Anestezi Dergisi, cilt.31, sa.4, ss.277-282, 2023 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 31 Sayı: 4
- Basım Tarihi: 2023
- Doi Numarası: 10.54875/jarss.2023.05900
- Dergi Adı: Anestezi Dergisi
- Derginin Tarandığı İndeksler: Scopus, Academic Search Premier, Central & Eastern European Academic Source (CEEAS), EMBASE, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.277-282
- Anahtar Kelimeler: Akciğer, kök hücre, Lung, propofol, propofol, stem cell
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objective: Lung mesenchymal stem cells (LMSCs) play important roles in the maintenance of organ integrity and healing processes. It is important not to harm or decrease LMSCs, especially in patients with lung diseases. Propofol may be administered to patients with acute or chronic lung diseases due to the need for anesthesia or sedation. We aimed to investigate the cytotoxic/ proliferative effects of propofol on LMSCs. Methods: The mesenchymal stem cells were subjected to propo-fol at 25 μΜ (Group P25), 50 μΜ (Group P50), 100 μΜ (Group P100), or 0 μΜ (Group P0). The values of the cell prolifération curve and the normalized cell index at the 6th, 24th, 48th, and 72nd hours after propofol administration were obtained with the xCEL-Ligence Real-Time Cell Analyzer system. Results: The normalized cell index values were lower in Group P100 compared to Group P25 at the 6th, 24th, 48th, and 72nd hours. They were also lower in Group P100 compared to Group P0 (p=0.001) at the 24th hour, but this effect was not seen at the fol-lowing time points. Conclusion: The cytotoxic effect of 100 μΜ propofol was obser-ved only after 24 hours and this effect was temporary. Therefore, propofol can be used during anesthesia practices, but we recom-mend using the lowest dose possible.