The Effects of Levetiracetam on Neural Tube Development in the Early Stage of Chick Embryos
Turkish Neurosurgery, cilt.23, sa.5, ss.617-622, 2013 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 23 Sayı: 5
- Basım Tarihi: 2013
- Doi Numarası: 10.5137/1019-5149.jtn.7471-13.0
- Dergi Adı: Turkish Neurosurgery
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.617-622
- Anahtar Kelimeler: Levetiracetam, Chick embryo, Neural tube defect
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Aim: This study aimed to investigate the effects of a new generation antiepileptic agent, levetiracetam, on the neural tube development in a chick embryo model that corresponds to the first month of vertebral development in mammals. Ma terial and Methods: Forty-five atabey® breed fertilized chicken eggs with no specific pathogens were randomly divided into 5 groups. All of the eggs were incubated at 37.8±2oC and 60±5 % relative humidity in an incubator. Group A was control group. The other eggs were applied physiological saline and drugs at a volume of 10 μL by the in ovo method at the 28th hour of the incubation period. Group B was given distilled water; Group C, physiological saline; Group D, Levetiracetam (L8668) at a dose equivalent to the treatment dose for humans (10 mg/ kg), and Group E, Levetiracetam (L8668) at a dose of 10 times the treatment dose. The embryos in all of the groups were removed from the shells at the 48th hour and morphologically and histologically evaluated. Results: Of the 45 embryos incubated, neural tubes of 41 were closed and the embryos displayed normal development. Conclusion: Levetiracetam, at a dose equivalent to human treatment dose and 10 times the treatment dose, was shown not to cause neural tube defects in chick embryos.