Determination of intracellular cytokines produced by Th1 and Th2 cells using flow cytometry in patients with brucellosis
FEMS Immunology and Medical Microbiology, cilt.45, sa.2, ss.253-258, 2005 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 45 Sayı: 2
- Basım Tarihi: 2005
- Doi Numarası: 10.1016/j.femsim.2005.04.001
- Dergi Adı: FEMS Immunology and Medical Microbiology
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.253-258
- Anahtar Kelimeler: brucellosis, intracellular cytokine, interferon-gamma, IL-4
- Sağlık Bilimleri Üniversitesi Adresli: Hayır
Özet
The aim of the study was to evaluate intracellular interferon-γ (IFN-γ), and interleukin-4 (IL-4) levels in pre- and post-treatment periods of brucellosis patients and to determine the relationship between these parameters and patients' clinical findings. Twenty-five patients diagnosed as brucellosis and 11 aged-matched healthy volunteers were included in the study. CD3+CD4+ T lymphocytes levels were significantly lower in patients with brucellosis as compared to the control group. CD3 +CD8+ T lymphocytes and CD3+IFN- γ+ levels were increased in brucellosis patients compared with the control group. CD4+IFN-γ+ and CD4 +IL-4+ levels were no different between patients and healthy individuals. CD3+IL-4+ levels decreased in patients compared with healthy controls. Pre-treatment CD3+IFN- γ+ levels dramatically increased in patients responsive to management compared with the unresponsive ones. In responsive cases, CD3 +IFN-γ+ levels decreased statistically after the treatment while in unresponsive cases no meaningful change was observed with respect to treatment. Adding IFN-γ to the treatment for improving the depleted levels of IFN-γ can be beneficial in patients with brucellosis who shows a tendency to chronicity or patients who do not respond to the treatment. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.